Jane made his film debut in the Telugu film Padamati Sandhya Raagam (1986), directed by late Jandhyala. His early roles included Zeph in Buffy the Vampire Slayer (1992). He had supporting roles in several high profile films, including The Crow: City of Angels (1996) Boogie Nights (1997), The Thin Red Line (1998), Thursday (1998) and Magnolia (1999). After receiving critical acclaim as baseball player Mickey Mantle in 61*, Jane began receiving leading roles.

Jane and director Jonathan Hensleigh and Avi Arad have said in many interviews that Jane was the first and only actor to be asked to play the title role in the 2004 movie The Punisher. Jane initially turned down the role twice, as well as a part in the first X-Men movie and a few other comic book movies, mainly because he does not see himself as a superhero actor and nor does he have any real interest or liking for superhero characters. Jane said that when they asked him the second time to play the Punisher that what really