In early 2000, Rodriguez broke off a near engagement to a Muslim boyfriend, citing opposition to religious requirements he demanded of her, which included covering herself completely. At one time, Rodriguez dated her The Fast and the Furious co-star, Vin Diesel. In 2003, Rodriguez was photographed sharing a kiss with Kylie Minogue's boyfriend Olivier Martinez and the two were later spotted partying together on on Puff Daddy's yacht in the south of France.

In 2006, she "had her eye on" Irish actor and S.W.A.T. co-star, Colin Farrell. In July 2006, Rodriguez told UK's Cosmopolitan magazine that she was not gay, but had "experimented with both sexes." In November of 2006, her openly bisexual Bloodrayne co-star Kristanna Loken's comments to The Advocate were widely interpreted and reported by the media as an admission that the two were in a relationship. Asked about the relationship in April 2007, Kristanna Loken said, "We're great friends. She'll always remain a great, close frie